Gram's Crystal Violet Solution SDS (Safety Data Sheet)

SECTION 1 — CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

Gram's Crystal Violet Solution

Flinn Scientific, Inc. P.O. Box 219, Batavia, IL 60510 (800) 452-1261
Chemtrec Emergency Phone Number: (800) 424-9669

Signal Word
WARNING

Hazard class: Flammable liquids (Category 3). Flammable liquid and vapor (H226). Keep away from heat, sparks, open flames, and hot surfaces. No smoking (P210).

Hazard class: Serious eye damage or irritation (Category 2A). Causes serious eye irritation (H319).

Hazard class: Skin corrosion or irritation (Category 3). Causes mild skin irritation (H316)

Hazard class: Carcinogenicity (Category 2). Suspected of causing cancer (H351). Obtain special instructions before use (P201). Do not handle until all safety precautions have been read and understood (P202). Use personal protective equipment as required (P281).

Pictograms

SECTION 3 — COMPOSITION, INFORMATION ON INGREDIENTS

Component Name	CAS Number	Formula	Formula Weight	Concentration
Crystal violet Ethyl alcohol, denatured Ammonium oxalate Water Synonyms: Hucker formula	548-62-9 64-17-5 6009-70-7 7732-18-5	C₂5H₃₀ClN₃ C₂H₅OH (NH₄)₂C₂O₄•H₂O H₂O	408.00 46.07 142.11 18.00	1.4% 16.1% 0.8% >81.6%

SECTION 4 — FIRST AID MEASURES

If exposed or concerned: Get medical advice or attention (P308+P313).
If inhaled: Remove victim to fresh air and keep at rest in a position comfortable for breathing. If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do so. Continue rinsing (P305+P351+P338). If eye irritation persists eyes: Get medical advice or attention (P337+P313).If on skin or hair: Immediately remove all contaminated clothing. Rinse skin with water (P303+P361+P353). If skin irritation occurs: Get medical advice or attention (P332+P313).
If swallowed: Rinse mouth.Call a POISON CENTER or physician if you feel unwell.

SECTION 5 — FIRE FIGHTING MEASURES

Class IC flammable liquid due to ethyl alcohol content.
When heated, releases flammable fumes.
In case of fire: Use a tri-class dry chemical fire extinguisher (P370+P378).

NFPA Code

H-1
F-3
R-0

SECTION 6 — ACCIDENTAL RELEASE MEASURES

Remove all ignition sources and ventilate area. Contain the spill with sand or other inert absorbent material and deposit in a sealed bag or container. See Sections 8 and 13 for further information.

SECTION 7 — HANDLING AND STORAGE

Flinn Suggested Chemical Storage Pattern: Organic #9. Store with dyes, indicators and stains.
Store in a dedicated flammables cabinet. If a flammables cabinet is not available, store in Flinn Saf-Stor™ can. Keep container tightly closed (P233). Keep cool (P235). Ground or bond container and receiving equipment (P240). Use explosion-proof electrical and ventilating equipment (P241). Use only non-sparking tools (P242).

SECTION 8 — EXPOSURE CONTROLS, PERSONAL PROTECTION

Wear protective gloves, protective clothing, and eye protection (P280). Wash hands thoroughly after handling (P264). Will stain skin, clothing, and surfaces.
Exposure guidelines: (as ethyl alcohol) PEL 1000 ppm (OSHA), Ceiling 1000 ppm (ACGIH)

SECTION 9 — PHYSICAL AND CHEMICAL PROPERTIES

Purple-violet liquid. Alcohol odor.

Boiling point: 78 °C (ethyl alcohol)

Biological stain.
Gram stain recipe.

SECTION 10 — STABILITY AND REACTIVITY

Avoid any source of fire or ignition.
Shelf life: Good, if stored properly.

SECTION 11 — TOXICOLOGICAL INFORMATION

Acute effects: Eye irritation, nausea, dizziness, headache.
Chronic effects: Liver damage, reproductive, teratogenic effects, carcinogen.
Target organs: Eyes, skin, central nervous system, liver, reproductive system.

ORL-RAT LD₅₀: 7060 mg/kg (as ethyl alcohol)
IHL-RAT LC₅₀: 20,000 ppm/10H (as ethyl alcohol)
SKN-RBT LD₅₀: N.A.

SECTION 12 — ECOLOGICAL INFORMATION

Data not yet available.

SECTION 13 — DISPOSAL CONSIDERATIONS

Please review all federal, state and local regulations that may apply before proceeding.
Flinn Suggested Disposal Method #26b is one option.

SECTION 14 — TRANSPORT INFORMATION

Shipping name: Ethyl alcohol. Hazard class: 3, Flammable liquid. UN number: UN1170.

SECTION 15 — REGULATORY INFORMATION

Not listed.

SECTION 16 — OTHER INFORMATION

This Safety Data Sheet (SDS) is for guidance and is based upon information and tests believed to be reliable. Flinn Scientific, Inc. makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages relating thereto. The data is offered solely for your consideration, investigation, and verification. The data should not be confused with local, state, federal or insurance mandates, regulations, or requirements and CONSTITUTE NO WARRANTY. Any use of this data and information must be determined by the science instructor to be in accordance with applicable local, state or federal laws and regulations. The conditions or methods of handling, storage, use and disposal of the product(s) described are beyond the control of Flinn Scientific, Inc. and may be beyond our knowledge. FOR THIS AND OTHER REASONS, WE DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE OR EXPENSE ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE OR DISPOSAL OF THIS PRODUCT(S).

Consult your copy of the Flinn Science Catalog/Reference Manual for additional information about laboratory chemicals.

Gram's Crystal Violet Solution Safety Data Sheet (SDS)

Gram's Crystal Violet Solution